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Algal strain identification

The RCC offers the service of identifying algal strains using a combination of light microscopy and genetic barcoding analysis. The cost per strain for our basic identification service (light microscopy and barcoding using 18S (for eukaryotes) or 16S (for prokaryotes) rRNA analysis) is 50 € for public-sector users and 150 € for private-sector users. We also offer an advanced identification service for eukaryotes comprising light microscopy, 18S analysis, as well as ITS, 28S or plastidial 16S barcoding for better phylogenetic resolution. The advanced identification service costs 100€ for public-sector users and 200 € for private-sector users.

Algal strain identification The RCC offers the service of identifying algal strains using a combination of light microscopy and genetic barcoding analysis. The cost per strain for our basic

Molecular strain identification of the Mycobacterium tuberculosis complex in archival tissue samples

Affiliation

  • 1 Institute of Pathology, Academic-Teaching Hospital München-Bogenhausen, D-81925 München, Germany.
  • PMID: 15509681
  • PMCID: PMC1770489
  • DOI: 10.1136/jcp.2003.015719

Free PMC article

Molecular strain identification of the Mycobacterium tuberculosis complex in archival tissue samples

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Authors

Affiliation

  • 1 Institute of Pathology, Academic-Teaching Hospital München-Bogenhausen, D-81925 München, Germany.
  • PMID: 15509681
  • PMCID: PMC1770489
  • DOI: 10.1136/jcp.2003.015719

Abstract

Aims: To investigate the use of different molecular analyses that can identify distinct strains of human pathogenic mycobacteria in formalin fixed and paraffin wax embedded archival tissue samples to see whether it is possible to differentiate between the members of the Mycobacterium tuberculosis complex (M tuberculosis, M bovis, M africanum, M microti, or M canettii) and/or substrains in a high number of samples. This would be of interest for identifying individual infection traits and superinfection by different mycobacterial strains.

Methods: Forty nine archival tissue samples with clinically and/or histologically suspected tuberculosis infection were subjected to molecular DNA analysis.

Results: The molecular analysis revealed the presence of M tuberculosis complex DNA in 20 samples, whereas acid fast bacilli could be detected by Ziehl-Neelsen staining in only eight samples. All IS6110 positive samples were further characterised by spoligotyping and seven cases provided M tuberculosis specific signatures, whereas M bovis specific signatures were obtained in four cases. The analysis of mtp40, oxyR, and pncA partial gene sequences confirmed the presence of M tuberculosis in six cases and M bovis in one case. The amplification and sequencing of four further genetic regions (katG, gyrA, TbD1, RD9) characterised six “modern” M tuberculosis strains belonging to genetic groups 2 or 3.

Conclusion: This study provides clear evidence that archival paraffin wax embedded material can be used for further studies on the strain identification of M tuberculosis complex strains and can therefore unequivocally be used for the study of the epidemiology and evolution of tuberculosis pathogens.

Figures

Polymerase chain reaction (PCR) amplification…

This study provides clear evidence that archival paraffin wax embedded material can be used for further studies on the strain identification of M tuberculosis complex strains and can therefore unequivocally be used for the study of the epidemiology and evolution of tuberculosis pathogens.