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Development of an in vitro protocol for a difficult-to-propagate endemic Australian dryland sedge species Mesomelaena pseudostygia (Cyperaceae)

Abstract

In vitro propagation for Mesomelaena pseudostygia a difficult-to-propagate dryland sedge species (Cyperaceae) endemic to Western Australia is described. Multiple avenues to in vitro propagation were investigated: shoot culture, organogenesis and somatic embryogenesis, with zygotic embryos as initiation material. The highest multiplication rate for shoots was 3.4 ± 1.0 after 6 wk on basal medium (1/2 strength Murashige and Skoog) with 2.5 μM kinetin and 0.5 μM 6-benzylaminopurine. Shoots achieved peak rooting (83%) following a pulse treatment on basal medium containing 10 μM indolebutyric acid and 2 μM α-naphthaleneacetic acid for 7 wk, followed by transfer to medium (without growth regulators) for a further 7 wk. Alternatively, in vitro grown shoots were pulse treated on basal medium with both 100 μM indolebutyric acid and 20 μM α-naphthaleneacetic acid for 1 wk then placed in Rockwool plugs (under propagation house conditions) for another 7 wk resulting in 63% root induction. Rooted plantlets were also successfully transferred to potting mixture either in Rockwool plugs or bare rooted and maintained in propagation house conditions with ≥95% survival after 7 wk. These results indicate that micropropagation of M. pseudostygia is feasible for small to medium scale restoration purposes. The highest frequency of callus induction was from cultured zygotic embryos on basal medium with 5 μM α-naphthaleneacetic acid, whereas 2,4-dichlorophenoxacetic acid (2 or 5 μM) produced the largest callus sizes. A low frequency of shoot regeneration occurred in zygotic callus tissues in basal medium treatments containing cytokinin (kinetin or thidiazuron at 1 μM). A small proportion (

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In vitro propagation for Mesomelaena pseudostygia a difficult-to-propagate dryland sedge species (Cyperaceae) endemic to Western Australia

Radula marginata seeds

Quote:
karode13 said:
What I typed applies to Australia.

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At first sight, The Perfection of Wisdom is bewildering, full of paradox and apparent irrationality.

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At first sight, The Perfection of Wisdom is bewildering, full of paradox and apparent irrationality.

and how long it takes to cultivate, i fear that it is very slow growing and would take too long to grow.

where have you seen Griffonia simplicifolia trees for sale?
I want to buy some seeds but have not been able to find anyone who has been able to grow it from seed.
I thought they were bringing out a law over the ditch that will make 5htp illegal.

Edited by Cactusdan (07/19/10 10:21 AM)

Quote:
inski said:
The new cannabinoid found in R. marginata is perrottetinenic acid which has a similar molecular structure to Delta(1) tetrahydrocannabinol and was published in 2002, perrottetinene is an already known cannabinoid found in this and other species of Radula, in particular, R. perrottetii.
It is suspected that perrottetinene is an active cannabinoid agonist but has yet to be proven, it has been successfully synthesized.
The quantities of these cannabinoids found in the ether extracts of Radula species are minor!
inski.

Anyone have anymore information about these fytocannabinoïds? It produces perrottetinene, perrottetinenic acid, and a couple other bibenzyl cannabinoids I cant find anything on.

Anyone else know a plant other than cannabis and Radula Marginata that produces cannabinoids?

Any papers revolving:
growth of the plant?
alkaloid-content (I am aware that cannabinoids contain no nitrogen group so technically they can’t be called alkaloids)
synthesis?
Pharmacology in human subject or does anybody have any experience over here? Has anyone grown/extracted?

Edited by Beanhead (06/16/11 02:22 AM)

B. Cannabinoids from Radula Plants
It was presumed that cannabinoids are unique to the Cannabis plant. However, the bibenzyl
cannabinoid, perrottetinene, was isolated in 1994 from the small leafy-stem liverwort, Radula
perrottetii. The same compound was found in Radula laxiramea. Toyota isolated
perrottetinene and its acid, perrottetinenic acid in the New Zealand liverwort Radula marginata.

Medicinal Research Reviews DOI 10.1002/med

C’mon now that’s almost 17 years since it’s isolation, has there been no spawn of new information about it?

I’m able to find a few snippets here and there about it but it isn’t much. The two papers I checked that is all the information they could supply.

I did however come across a gem for synthesis. ->attached file.
Here’s another one so if anyone that has access to it. pubs

Edited by Beanhead (06/16/11 02:23 AM)

I just stumbled upon basic info for this plant! Now I want to know more.

has any more info come in since a year ago’s last post?

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Sit up and meditate, there’s no time to contemplate.
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I have an international Hitech Psytrance project with a friend: BioChronic
I make various form of Psytrance as a solo Project Dendriform

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very interesting Perrottetinene, how do you know its non-toxic? or is it just the fact that it has not been studied???? There could be medical use for Perrottetinene as it is similar to THC. more