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Polyploidism and Superior Quality in Medical Marijuana by Billy Budd
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Origins of Pink Kush, OG Kush, Chemdawg:
During my research of polyploid cannabis. I have come up with what I believe to be the initial source of this super weed. It was said, but not confirmed, that UBC Professor David Suzuki, doing genetic research (1977), created a polyploid plant (tetraploid) by treating it with colchicine based upon the work of Menzel/Brown and Warmke. This plant was originally a hybrid of Thai (sativa) x Purple Afghani (indica) and was then treated with Colchicine (Autumn Locus bulb). There are certainly other clones that were created by other breeders, especially in Hawaii, but the initial commercial tetraploid was developed in B.C. by the Canadian Government and UBC for Cancer patients. I would estimate that the THC content of polyploids to be well over 30% and maybe up to 40% if grown correctly. Fully grown mother tetraploid plants have been known to sell for $10,000. Clones have been known to sell for $1,000 in the past compared to the standard $5-7 range for diploid clones. Tetraploids are extremely difficult to clone (10-20%) unless special care is taken. The end product is far superior to anything commercially available and even compared to excellent connoisseur diploid product due to the doubling of THC. See my Secrets of the Pink Kush book for updated information on this and more topics.
The resulting F1 hybrid seeds were then grown out and used for cancer patients at UBC. Allegedly somebody stole one of the clones that the Canadian Government and David Suzuki, supposedly, had created. It became famous and was then re-created by treating other plants and sold out east as Diesel and as OG Kush on the west coast and as ChemDawg in Colorado. These were all colchicine treated plants based upon their work and the popularity of UBC Chemo. Please note that, according to Sam the Skunkman AKA David Watson, David Suzuki denies that he created this plant. Please note that David Suzuki has always been a government employee and as such his veracity cannot be completely trusted in this world of ours. He would deny it simply because he would lose his job. I do not face that fear.
My understanding is that some clones were stolen. This would make more sense. Diploid and Tetraploid crosses produce Triploids that are infertile due to chromosome mismatch. These are known as “Terminator Seeds” (e.g. Diablos Pink Kush) as customers must buy seeds for each crop due to infertility. The resulting generations of seeds will be diploid. Triploids can be treated again, with Colchicine, to create a Hexaploid. This can be then crossed with a tetraploid or diploid to produce tetraploid plants again. I believe that Warmke was wrong about triploids and Sam the Skunkman thinks that they are not sterile.
How To Identify Polyploids:
You can identify a polyploid be mere physical appearance and no need to examine the chromosones. Most people have never grown or smoked these real legendary plants. The gangters rap about OG Kush because they had the money to buy this extremely rare connoisseur product. Most product out there is just “wangsta OG”.
- Four sets of leaves at the nodes
- Stretched (doubled) flowers
- Dark green leaves
- Double bud sites
- Heavy feeder
- High water consumption
- Thick meristem
- Pistil discoloration (pink pistils under black light)
- Extreme potency
- Crooked looking plant providing a Bonsai appearance
- Larger stomata
- Thicker leaves
- NOTE: In order to correctly determine a polyploid level you must extract DNA from the root stain the sample (several methods), put under microscope, count the gametes surrounding the nuclei. You must draw what you see in the microscope and then count. As cannabis has 10 gametes, the number should be 20 for 2n (normal diploid), 40 for 4n (tetraploid), 30 for 3n (triploid), and 60 (hexaploid) for 6n and 80 for 8n (octoploid).
- Secrets of the Pink Kush – Advanced Knowledge of Polyploids in plants, insects, animals and humans.
If your plant (OG Kush or Chemo or Pink Kush or Chem Dawg or Master Kush) does not have four sets of leaves at the nodes then it is NOT a true polyploid plant and it not the real deal. UBC Chemo is a polyploid and had four leaf internodes. These tetraploid cuttings have different names but are believed to all be the famous polyploids named OG Kush, Pink Kush, Chemdawg, Diesel, Master Kush etc. These are really heavy feeders due to the double size buds and double the number of leaves and buds and THC glands. This plant is very strange and has a chemical smell to it. UBC Chemo, for example, has a Haze and Kush taste due to the Thai sativa and Afghani indica influences. Polyploid plants are almost twice as strong as diploid plants due to the doubling of THC molecules. These plants are extremely difficult to clone and grow very slowly due to the doubling of leaves unless fed correctly. Water consumption is extremely high. If it only has two leaves then it is a normal diploid and will not have the same effect as the three molecule THC of a polyploid. Three leaves does not necessarily mean it is polyploid as it could be just whorled phyllotaxis (just created by stress, removing leaves and the subsequent regeneration of leaves is deformed). Four leaves does mean polyploid. Triploids, being sterile, must be treated to create hexaploids. These hexaploids can then be used to create seeds that will be polyploids.
WARNING: Colchicine is a extremely toxic alkaloid and is highly poisonous. Long term contact can result in white blood cell anomolies. You cannot smoke a plant that has been treated, post germination, with Colchicine. You can only consume the grown out product of the seeds. You can smoke product that was treated prior to germination. Selective breeding is not genetically modified cannabis. This is genetically modified pot as it was altered at the chromosonal level. Never treat a plant with Colchicine as it will become toxic and will eventually revert to diploid with further treatment. You are changing the molecular structure dynamically and none of the product can be consumed. The correct way is to treat the seeds.
G-13 The story on this is that it was created by the University of Mississippi under the aegis of the infamous Carlton Turner. Apparently this cannot be true as U of M never did genetic engineering. If you Google G-13 you will find it is made by Suzuki. Well David Suzuki did create a GM Genetically Modified plant – Chemo. What letter is the 13th letter of the alphabet? M. So we have G-13 or GM for genetically modified and made by Suzuki. Sounds like an great code name to me. I strongly suspect that G-13 is just the Chemo clone and yet another name for it. UPDATE May 11, 2014: David Suzuki denies ever having made that plant and an old friend of mine believes that the Chemo actually came from the University of Washington.
I was offered a cutting of the Chemo plant in Ontario back in the mid 1980’s. I had been transferred from B.C. when the recession hit. Several other people from B.C. went to Ontario as well in search of work. We brought out seeds, clones and technology with us. There was a guy who ran a Hydroponics store in Toronto that had the Chemo clone for sale. I cannot remember the price but it was FAR too much money. I did not comprehend the value of this plant at that time and thought he was crazy. Now, after having seen it, I understand.
- UBC Chemo
- OG Kush
- Master Kush
- Pink Kush
- Acapulco Gold (suspected polyploid, long gone)
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Polyploid Cannabis. What is polyploid cannabis?
Polyploid cannabis is a chromosome-related variation of normal cannabis. It offers the possibility of heavier harvests and stronger effects. To understand polyploid cannabis we need to start by looking at chromosomes. A chromosome is made of DNA. It contains part, or all, of the genetic material for that organism. Humans have 23 pairs of chromosomes, one from each parent. In humans there are two paired chromosomes in each set, this is know as diploidy. Cannabis is also a diploid species. But if you have three or more sets of chromosomes (instead of two) the organism is said to exhibit polyploid characteristics. Polyploid cannabis would have 3, or more, paired chromosomes instead of the normal two.
What is polyploid cannabis. Will polyploid cannabis have more THC?
Cannabis, like humans, usually shows diploid behaviour. Normally two chromosomes are present in each pair, one set of chromosomes is inherited from each parent. This means that the dominant characteristics of a particular cannabis variety are inherited from the parents. This is the basis of cannabis breeding. However, some professional research work has been ongoing to see what benefits polyploid cannabis would offer. Specifically the work is looking at differences between normal female and polyploid female cannabis plants during bloom. Investigations will focus on many aspects including comparative ease of growth, cannabinoid content, terpene content and harvest quantities.
Polyploid cannabis seeds. The biggest new development since feminized cannabis seeds?
Polyploid cannabis seeds represent a completely new approach to cannabis growing. Several years of laboratory work would be required to create, stabilise and assess the polyploid results. There are several advantages and disadvantages to polyploidy in plants. One of the main reasons why there is such huge interest in creating polyploid cannabis is to increase plant vigor (heterosis). This could improve yields and potency of the cannabis crops. The extra set (or sets) of cannabis chromosomes may allow for different growth rates and higher cannabinoid content. These days, cannabis is a multi $billion crop with huge recreational and medical markets. Any methods to further improve potency and yields are of serious interest. Those with most to gain are probably the largest commercial cannabis growers, where even small improvements to potency and yield can add $millions to revenue. If polyploid cannabis seeds can be produced at affordable prices they would also be of real interest to cannabis home growers. Most home growers only buy a few cannabis seeds each year and tend to focus on getting the highest yields and highest potency from the minimum number of plants. So polyploid cannabis seeds will be just as appealing to home growers as they would be to legal licensed producers.
Are polyploid cannabis seeds available yet?
Work has been ongoing for some time to create stable polyploid cannabis genetics. If the work is successful it is quite likely that polyploid cannabis seeds will become available in the coming years. Initially it is thought that professional cannabis growers in place like e.g. North America will be most interested in polyploid seeds. Just a few percentage points added to the annual yields or average crop potency can be the difference between becoming a market leader or losing market share. Researchers hope that polyploid cannabis seeds will produce plants with a higher cannabinoid content and heavier yields than non-polyploid cannabis seeds. If that proves to be the case then feminized polyploid cannabis seeds will become the new benchmark for quality. Though it is worth advising caution, and saying that any production of polyploid cannabis seeds is likely to be in relatively modest quantities. At least initially. If polyploid cannabis has significant advantages over traditional cannabis there will be huge interest from growers of all kinds.
Will polyploid seeds be the next cannabis seed revolution?
Originally only regular cannabis seeds existed. These produce male and female plants in equal quantities. Feminized cannabis seeds were first created by Dutch Passion in the 1990’s. At the time feminized seeds were seen as a revolution in convenience for growers. Nowadays, feminized cannabis seeds are seen as the standard and are used by most growers. Autoflowering cannabis seeds have been a success in more recent years, making cannabis growing easier and faster for millions of indoor and outdoor cannabis growers. CBD-rich cannabis seed varieties were developed and have proved incredibly useful, especially for medical cannabis growers. Selective breeding is currently underway to bring new varieties enriched in cannabinoids such as THC-V and CBG. Cannabis breeders and professional seed companies are investing heavily and working hard to squeeze every possible benefit out of our beloved cannabis plant. If successful, polyploid cannabis seeds could be the next major evolution in the world of cannabis growing. Scientific interest in polyploid cannabis has been gathering pace as the value of medical/recreational cannabis markets soar. This research paper gives some technical insight into the subject. The Robert Connell Clarke book ‘Polyploid Review’ can be read online here .
Is Polyploid cannabis the same as genetically modified cannabis?
No. These are two clearly separate technologies. So far no-one has so far made a form of genetically modified (GM) cannabis. Creating a Polyploid organism is a completely different technical approach. Genetically modified crops are known as GM Crops or biotech crops. Making a GM crop means complicated modifications to the DNA using expensive high-tech genetic engineering. This has been done for food crops to increase resistance to pest, drought etc. GM crops now account for 12% of global crops and and includes crops such as Maize and Soybean, but not cannabis.
Polyploid cannabis – is it natural?
Polyploidism occurs in nature. Plant species such as wheat are a good example of natural polyploidy. Wheat has been bred by humans for thousands of years and breeding has resulted in diploid, tetraploid and hexaploid crops (6 sets of paired chromosomes). Durum wheat is a tetraploid and common bread wheat is a hexaploid. Tetraploidy in Durum wheat is the result of the hybridization of two diploid grass species. Durum wheat contains two sets of chromosomes derived from one parent and two sets of chromosomes from the other. Polyploid plants are no more dangerous than any others.
Polyploid cannabis and how it can be induced.
One method is to use the chemical Colchicine . This is found in the Autumn Crocus and is an anti-inflammatory used to treat Gout in humans. Colchicine can be dissolved in water and used to soak cannabis seeds to induce polyploidy. Or it can be applied to growing cannabis plants as a gel. Colchicine induces polyploidy in plant cells by preventing separation of chromosomes during cell division. The result is that half of the resulting (gamete) cells have no chromosomes and the other half have double. Inducing polyploidy is one thing. Being able to control, stabilize and use the benefits productively is another.
Polyploid cannabis. Work continues.
If the polyploid research on cannabis goes well the implications will be huge for the cannabis industry. Stabilising polyploidy and creating feminized polyploid cannabis seeds will require even more work. If polyploid cannabis can increase either yield or potency then it will be of great interest to the entire cannabis industry. Stay tuned!Read on to find out more about polyploid cannabis. Polyploid cannabis is being researched to see if it can improve yield or potency of the final harvest. ]]>