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Poly seeds

Does anyone here — besides me, that is — use the phrase “polly
seeds” instead of “sunflower seeds”? If so, I’d be curious to know you
lived when you started using that term.

Dena Jo

Email goes to denajo2 at the dot com variation of the Yahoo domain.

Plonk the bastards: http://www.panix.com/

COMING SOON to a news server near you!
Misc.writing.screenplays.moderated: Just like MWS, only better.
View the trailer here: http://www.panix.com/

Dena Jo wrote:
Does anyone here — besides me, that is — use the phrase
“polly seeds” instead of “sunflower seeds”? If so, I’d be
curious to know you lived when you started using that term.

Is it “polly seeds” or “poly seeds”?. I am guessing that usage
spread from New York City. Is there a term that sounds like
that in another language? What we have so far is a possible
favorite food of parrots.

When I was growing up, everyone in my family called them polly seeds.
After we moved to L.A., we all switched to calling them sunflower
seeds. I’d always assumed it was a New York, maybe even a Brooklyn,
thing.

Email goes to denajo2 at the dot com variation of the Yahoo domain.

Plonk the bastards: http://www.panix.com/

COMING SOON to a news server near you!
Misc.writing.screenplays.moderated: Just like MWS, only better.
View the trailer here: http://www.panix.com/

(Warcloud):
Barbaric policies, slaughter you in the odyssey
Crack you like a Polly seed, make your organs come out of thee
Shot ’em barefoot in the dark, you was a tyrant

” . and if they could see me they could see something lovely and not
keep laughing at me , throunging spitballs . and Polly seed shells at
me “

“My father used to tell funny stories about how the guys used to try to
outwit the army. One thing was to spend the whole month eating polly
seeds (sunflower seeds) and herring. The result was your blood pressure
went up a lot”

“Hymie’s
Allerton and Holland Aves
Approximate dates: 1940-50
Sacks of nuts stacked on the floor. Delicious candy showcase. Sold bags
of 5 cent polly seeds, malted, cherry lime rickys (made from real
squeezed limes!) and loose cigarettes.”

“In front of the luncheonette were 2 penny nut machines one for
“polly-seeds”, (sunflower seeds), and the other for Indian nuts, and
there was a bench where one would find daily newspapers.”

Couple dozen more Googlehits for “polly seeds”. Seems to be a NY thang,
like you said.
Now we have to know why they were called polly seeds.

Poly seeds Does anyone here — besides me, that is — use the phrase “polly seeds” instead of “sunflower seeds”? If so, I’d be curious to know you lived when you started using that term. — Dena

Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds

Affiliations

  • 1 Metabolix Oilseeds Inc, Saskatoon, SK, Canada.
  • 2 Donald Danforth Plant Science Center, Saint Louis, MO, USA.
  • 3 Metabolix Inc, Cambridge, MA, USA.
  • 4 Department of Biological Sciences, Webster University, Saint Louis, MO, USA.
  • 5 Department of Biology, Washington University, Saint Louis, MO, USA.
  • 6 Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska, Lincoln, NE, USA.
  • PMID: 25418911
  • DOI: 10.1111/pbi.12290

Free article

Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds

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Authors

Affiliations

  • 1 Metabolix Oilseeds Inc, Saskatoon, SK, Canada.
  • 2 Donald Danforth Plant Science Center, Saint Louis, MO, USA.
  • 3 Metabolix Inc, Cambridge, MA, USA.
  • 4 Department of Biological Sciences, Webster University, Saint Louis, MO, USA.
  • 5 Department of Biology, Washington University, Saint Louis, MO, USA.
  • 6 Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska, Lincoln, NE, USA.
  • PMID: 25418911
  • DOI: 10.1111/pbi.12290

Abstract

Poly-3-hydroxybutyrate (PHB) production in plastids of Camelina sativa seeds was investigated by comparing levels of polymer produced upon transformation of plants with five different binary vectors containing combinations of five seed-specific promoters for expression of transgenes. Genes encoding PHB biosynthetic enzymes were modified at the N-terminus to encode a plastid targeting signal. PHB levels of up to 15% of the mature seed weight were measured in single sacrificed T1 seeds with a genetic construct containing the oleosin and glycinin promoters. A more detailed analysis of the PHB production potential of two of the best performing binary vectors in a Camelina line bred for larger seed size yielded lines containing up to 15% polymer in mature T2 seeds. Transmission electron microscopy showed the presence of distinct granules of PHB in the seeds. PHB production had varying effects on germination, emergence and survival of seedlings. Once true leaves formed, plants grew normally and were able to set seeds. PHB synthesis lowered the total oil but not the protein content of engineered seeds. A change in the oil fatty acid profile was also observed. High molecular weight polymer was produced with weight-averaged molecular weights varying between 600 000 and 1 500 000, depending on the line. Select lines were advanced to later generations yielding a line with 13.7% PHB in T4 seeds. The levels of polymer produced in this study are the highest reported to date in a seed and are an important step forward for commercializing an oilseed-based platform for PHB production.

Keywords: Camelina sativa; bioplastics; oilseeds; poly-3-hydroxybutyrate; polyhydroxyalkanoate; polyhydroxybutyrate.

© 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

Poly-3-hydroxybutyrate (PHB) production in plastids of Camelina sativa seeds was investigated by comparing levels of polymer produced upon transformation of plants with five different binary vectors containing combinations of five seed-specific promoters for expression of transgenes. Genes encoding …